We host Monica Delay, US Manager for Technical Application Support at Cytek Biosciences, for a discussion on autofluorescence (AF) and the tools to deal with it in Spectral Flow Cytometry. We use figures to showcase some problems with AF which will be missing from this audio version of our talk. You can visit our website at Chugcytometry.com to find the video, along with a list of links to webinars and publications dealing with AF.