In this episode of Practical Biotech, I take a technique focused approach to our typical episodes and provide you a detailed look at a widely used biochemical method. The ELISA is used in research, biotech, quality, and medicine and can be used to test for and identify a variety of large molecules. It is very sensitive due to its use of antibodies. You may have learned about this method in school but may not actually have any hands on time with it. In Technique Focus, I take you through the specifics of the method itself in order to give you an understanding of how its performed but to also illustrate how important it is in the biotech setting. Hopefully, you can take this understanding and build upon it to prepare you for working in industry post academic life.